pet23a ubc9 addgene (Addgene inc)
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pet23a ubc9 addgene
Pet23a Ubc9 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28b+addgene/pET28b-hUba2-His+(Plasmid+%2353117)/10__1038_slash_s44318___025___00532___y-257-18-19
Average 93 stars, based on 3 article reviews
Pet23a Ubc9 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28b+addgene/pET28b-hUba2-His+(Plasmid+%2353117)/10__1038_slash_s44318___025___00532___y-257-18-19
Average 93 stars, based on 3 article reviews
pet23a ubc9 addgene - by Bioz Stars,
2026-10
93/100 stars
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shRNA:Article Title: ARHGAP12 suppresses F-actin assembly to control epithelial tight junction mechanics and paracellular leak pathway permeability. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide (2%) Electron Microscopy Sciences Cat# 19152 Potassium Ferricyanide Sigma-Aldrich Cat# 702587 Low Molecular Weight Tannic Acid Electron Microscopy Sciences Cat# 21700 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Lead Citrate Trihydrate Electron Microscopy Sciences Cat# 17800 Synthesized peptide NP1: RRQAPPPPPPSRGGP GenScript N/A Synthesized peptide NP1/2: RRQAPPPPPPSR GGPPPPPPPPHNSGPPPPPARGRGA GenScript N/A Synthesized peptide NP1/2/3: RRQAPPPPPPSRGGPPPPPPPPHN SGPPPPPARGRGAPPPPPSRAPTA GenScript N/A Synthesized peptide NP3/4/5: GRGAPPPPPSRAPTAAPPPPPP SRPSVAVPPPPPNRMYPPPPPALPSS GenScript N/A Synthesized peptide NP1 (R/A) mutant: RRQAPPPPPPSAGGP GenScript N/A Synthesized Mena peptide: GPPPPPPLPSTGPPP GenScript N/A Blebbistatin Sigma-Aldrich Cat# 203391 CK-666 MedChemExpress Cat# HY-16926 CK-869 MedChemExpress Cat# HY-16927 SMIFH2 MedChemExpress Cat# 340316-62-3 DMEM, high glucose Gibco Cat# 21068028 DMEM, high glucose, no glutamine, no calcium Gibco Cat# 21068028 Fluorescein sodium salt Sigma-Aldrich Cat# F6377 Fluorescein isothiocyanate–dextran (average mol wt 4,000) Sigma-Aldrich Cat# 46944 Fluorescein isothiocyanate–dextran (average mol wt 10,000) Sigma-Aldrich Cat# FD10S Fluorescein isothiocyanate–dextran (average mol wt 70,000) Sigma-Aldrich Cat# 46945 GFP-Trap Agarose Beads Chromotek Cat# gta-20 Paraformaldehyde Sigma-Aldrich Cat# 158127 PierceTM Protease Inhibitor Tablets, EDTA-free Thermo Fisher Scientific Cat# A32965 Polyethylenimine, Linear, MW 25000, Transfection Grade‘ Polysciences Cat# 23966-2 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat# H-1000-10 DAPI Sigma-Aldrich Cat# D9542 Phalloidin–Atto 565 Sigma-Aldrich Cat# 94072 Experimental models: Cell lines MDCK-II cells ATCC CCLV Cat# CCLV-RIE 1061; RRID: CVCL_0424 MDCK-II ARHGAP12 KO cells This study N/A MDCK-II ZO-1 KO cells Provided by Walter Hunziker N/A MDCK-II ZO-2 KO cells Provided by Walter Hunziker N/A HEK293T cells ATCC RRID: CVCL_0063 COS7 cells ATCC CLS Cat# 605470; RRID: CVCL_0224 Oligonucleotides ARHGAP12 gRNA For: CACCGTCTCATGCCGCCTGTTAAAC Integrated DNA Technologies, (IDT) N/A ARHGAP12 gRNA Rev: AAACGTTTAACAGGCGGCATGAGAC Integrated DNA Technologies, (IDT) N/A N-WASP shRNA For: /5Phos/GATCCCCGCAGCAGATC GTAACTGTATTCAAGAGATACA GTTACGATCTGCTGC TTTTTA Integrated DNA Technologies, (IDT) N/A (Continued on next page) Cell Reports 44, 115511, April 22, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-WASP shRNA Rev: /5Phos/AGCTTAAAAAGCAGCAG ATCGTAACTGTATCTCTTGAATA CAGTTACGATCTGCTGC GGG Integrated DNA Technologies, (IDT) N/A See Table S1 for all other primers and oligonucleotides N/A Recombinant DNA ARHGAP12 Source BioScience IRATp970H02100D IMAGE clone ID #30528830 NCBI: BC094719 LifeAct-mCherry Addgene Cat# 193300 pEGFP C1 Addgene Cat# 13031 pmCherry C1 Clontech Cat# 632524 pET28b( Recombinant:Article Title: ARHGAP12 suppresses F-actin assembly to control epithelial tight junction mechanics and paracellular leak pathway permeability. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide (2%) Electron Microscopy Sciences Cat# 19152 Potassium Ferricyanide Sigma-Aldrich Cat# 702587 Low Molecular Weight Tannic Acid Electron Microscopy Sciences Cat# 21700 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Lead Citrate Trihydrate Electron Microscopy Sciences Cat# 17800 Synthesized peptide NP1: RRQAPPPPPPSRGGP GenScript N/A Synthesized peptide NP1/2: RRQAPPPPPPSR GGPPPPPPPPHNSGPPPPPARGRGA GenScript N/A Synthesized peptide NP1/2/3: RRQAPPPPPPSRGGPPPPPPPPHN SGPPPPPARGRGAPPPPPSRAPTA GenScript N/A Synthesized peptide NP3/4/5: GRGAPPPPPSRAPTAAPPPPPP SRPSVAVPPPPPNRMYPPPPPALPSS GenScript N/A Synthesized peptide NP1 (R/A) mutant: RRQAPPPPPPSAGGP GenScript N/A Synthesized Mena peptide: GPPPPPPLPSTGPPP GenScript N/A Blebbistatin Sigma-Aldrich Cat# 203391 CK-666 MedChemExpress Cat# HY-16926 CK-869 MedChemExpress Cat# HY-16927 SMIFH2 MedChemExpress Cat# 340316-62-3 DMEM, high glucose Gibco Cat# 21068028 DMEM, high glucose, no glutamine, no calcium Gibco Cat# 21068028 Fluorescein sodium salt Sigma-Aldrich Cat# F6377 Fluorescein isothiocyanate–dextran (average mol wt 4,000) Sigma-Aldrich Cat# 46944 Fluorescein isothiocyanate–dextran (average mol wt 10,000) Sigma-Aldrich Cat# FD10S Fluorescein isothiocyanate–dextran (average mol wt 70,000) Sigma-Aldrich Cat# 46945 GFP-Trap Agarose Beads Chromotek Cat# gta-20 Paraformaldehyde Sigma-Aldrich Cat# 158127 PierceTM Protease Inhibitor Tablets, EDTA-free Thermo Fisher Scientific Cat# A32965 Polyethylenimine, Linear, MW 25000, Transfection Grade‘ Polysciences Cat# 23966-2 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat# H-1000-10 DAPI Sigma-Aldrich Cat# D9542 Phalloidin–Atto 565 Sigma-Aldrich Cat# 94072 Experimental models: Cell lines MDCK-II cells ATCC CCLV Cat# CCLV-RIE 1061; RRID: CVCL_0424 MDCK-II ARHGAP12 KO cells This study N/A MDCK-II ZO-1 KO cells Provided by Walter Hunziker N/A MDCK-II ZO-2 KO cells Provided by Walter Hunziker N/A HEK293T cells ATCC RRID: CVCL_0063 COS7 cells ATCC CLS Cat# 605470; RRID: CVCL_0224 Oligonucleotides ARHGAP12 gRNA For: CACCGTCTCATGCCGCCTGTTAAAC Integrated DNA Technologies, (IDT) N/A ARHGAP12 gRNA Rev: AAACGTTTAACAGGCGGCATGAGAC Integrated DNA Technologies, (IDT) N/A N-WASP shRNA For: /5Phos/GATCCCCGCAGCAGATC GTAACTGTATTCAAGAGATACA GTTACGATCTGCTGC TTTTTA Integrated DNA Technologies, (IDT) N/A (Continued on next page) Cell Reports 44, 115511, April 22, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-WASP shRNA Rev: /5Phos/AGCTTAAAAAGCAGCAG ATCGTAACTGTATCTCTTGAATA CAGTTACGATCTGCTGC GGG Integrated DNA Technologies, (IDT) N/A See Table S1 for all other primers and oligonucleotides N/A Recombinant DNA ARHGAP12 Source BioScience IRATp970H02100D IMAGE clone ID #30528830 NCBI: BC094719 LifeAct-mCherry Addgene Cat# 193300 pEGFP C1 Addgene Cat# 13031 pmCherry C1 Clontech Cat# 632524 pET28b( Software:Article Title: ARHGAP12 suppresses F-actin assembly to control epithelial tight junction mechanics and paracellular leak pathway permeability. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide (2%) Electron Microscopy Sciences Cat# 19152 Potassium Ferricyanide Sigma-Aldrich Cat# 702587 Low Molecular Weight Tannic Acid Electron Microscopy Sciences Cat# 21700 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Lead Citrate Trihydrate Electron Microscopy Sciences Cat# 17800 Synthesized peptide NP1: RRQAPPPPPPSRGGP GenScript N/A Synthesized peptide NP1/2: RRQAPPPPPPSR GGPPPPPPPPHNSGPPPPPARGRGA GenScript N/A Synthesized peptide NP1/2/3: RRQAPPPPPPSRGGPPPPPPPPHN SGPPPPPARGRGAPPPPPSRAPTA GenScript N/A Synthesized peptide NP3/4/5: GRGAPPPPPSRAPTAAPPPPPP SRPSVAVPPPPPNRMYPPPPPALPSS GenScript N/A Synthesized peptide NP1 (R/A) mutant: RRQAPPPPPPSAGGP GenScript N/A Synthesized Mena peptide: GPPPPPPLPSTGPPP GenScript N/A Blebbistatin Sigma-Aldrich Cat# 203391 CK-666 MedChemExpress Cat# HY-16926 CK-869 MedChemExpress Cat# HY-16927 SMIFH2 MedChemExpress Cat# 340316-62-3 DMEM, high glucose Gibco Cat# 21068028 DMEM, high glucose, no glutamine, no calcium Gibco Cat# 21068028 Fluorescein sodium salt Sigma-Aldrich Cat# F6377 Fluorescein isothiocyanate–dextran (average mol wt 4,000) Sigma-Aldrich Cat# 46944 Fluorescein isothiocyanate–dextran (average mol wt 10,000) Sigma-Aldrich Cat# FD10S Fluorescein isothiocyanate–dextran (average mol wt 70,000) Sigma-Aldrich Cat# 46945 GFP-Trap Agarose Beads Chromotek Cat# gta-20 Paraformaldehyde Sigma-Aldrich Cat# 158127 PierceTM Protease Inhibitor Tablets, EDTA-free Thermo Fisher Scientific Cat# A32965 Polyethylenimine, Linear, MW 25000, Transfection Grade‘ Polysciences Cat# 23966-2 VECTASHIELD Antifade Mounting Medium Vector Laboratories Cat# H-1000-10 DAPI Sigma-Aldrich Cat# D9542 Phalloidin–Atto 565 Sigma-Aldrich Cat# 94072 Experimental models: Cell lines MDCK-II cells ATCC CCLV Cat# CCLV-RIE 1061; RRID: CVCL_0424 MDCK-II ARHGAP12 KO cells This study N/A MDCK-II ZO-1 KO cells Provided by Walter Hunziker N/A MDCK-II ZO-2 KO cells Provided by Walter Hunziker N/A HEK293T cells ATCC RRID: CVCL_0063 COS7 cells ATCC CLS Cat# 605470; RRID: CVCL_0224 Oligonucleotides ARHGAP12 gRNA For: CACCGTCTCATGCCGCCTGTTAAAC Integrated DNA Technologies, (IDT) N/A ARHGAP12 gRNA Rev: AAACGTTTAACAGGCGGCATGAGAC Integrated DNA Technologies, (IDT) N/A N-WASP shRNA For: /5Phos/GATCCCCGCAGCAGATC GTAACTGTATTCAAGAGATACA GTTACGATCTGCTGC TTTTTA Integrated DNA Technologies, (IDT) N/A (Continued on next page) Cell Reports 44, 115511, April 22, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-WASP shRNA Rev: /5Phos/AGCTTAAAAAGCAGCAG ATCGTAACTGTATCTCTTGAATA CAGTTACGATCTGCTGC GGG Integrated DNA Technologies, (IDT) N/A See Table S1 for all other primers and oligonucleotides N/A Recombinant DNA ARHGAP12 Source BioScience IRATp970H02100D IMAGE clone ID #30528830 NCBI: BC094719 LifeAct-mCherry Addgene Cat# 193300 pEGFP C1 Addgene Cat# 13031 pmCherry C1 Clontech Cat# 632524 pET28b( |